GenElute^™96孔板血液基因组DNA纯化试剂盒

GenElute is a trademark of Sigma-Aldrich Co. LLC

纯化所得的基因组DNA可直接供以下下游应用使用：

• PCR
• 限制性酶切
• Southern印迹
• 克隆
• 酶促反应

首先，起始材料在室温下溶解在含有离液盐和蛋白酶K的裂解液中。之后加入乙醇以确保形成可使DNA结合到血液结合反应板的硅胶膜上的适当条件。洗涤结合的DNA以去除污染物后，在该试剂盒所含的血液洗脱溶液中洗脱高纯度基因组DNA。

在您收到该试剂盒后，请立即将血液结合反应板放置在4°C下储存。本试剂盒之中的其他成分可在室温下储存。

GenElute 96 Well Blood Genomic DNA Purification Kit allows for high throughput purification of pure genomic DNA from whole blood, buffy coat, or cultured cells. The kit contains the advantages of silica membrane technology and eliminates the need for expensive resins, alcohol precipitations and hazardous compounds such as phenol and chloroform.

The starting materials are lysed at room temperature in a lysis buffer which contains chaotropic salts in the presence of Proteinase K. The addition of ethanol ensures the appropriate conditions to bind the DNA to the silica membrane of the Blood Binding Plate. After washing the bound DNA to remove contaminants, the pure genomic DNA is eluted in the Blood Elution Solution included in the kit.

The purified genomic DNA is ready for use in downstream applications such as PCR, Southern blotting, or any kind of enzymatic reaction.