biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
C21, monoclonal
contains
15 mM sodium azide
species reactivity
mammals
technique(s)
indirect ELISA: 1:2,500, western blot: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... CALD1(800)
mouse ... Cald1(109624)
rat ... Cald1(25687)
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Immunogen
chicken caldesmon
Biochem/physiol Actions
The antibody is directed against an epitope near the carboxy-terminus, Ca2+/calmodulin and F-actin binding site of caldesmon. Using ELISA, the antibody is able to inhibit caldesmon binding to Ca2+/calmodulin and F-actin. By immunoblotting, the antibody shows broad species reactivity to non-muscle caldesmon of mammalian cells.
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J J Lin et al.
Hybridoma, 7(3), 273-288 (1988-06-01)
Monoclonal antibodies, C2, C9, C18, C21 and C23, against chicken gizzard caldesmon have been prepared and characterized. These antibodies reacted with gizzard caldesmon (150 KDa) by enzyme-linked immunosorbent assay and protein immunoblotting. Immunofluorescence microscopy with these antibodies on cultured gizzard
Erik Y Zhang et al.
The American journal of pathology, 164(2), 601-612 (2004-01-27)
Partial bladder outlet obstruction (PBOO) induces remodeling of urinary bladder smooth muscle (detrusor). We demonstrate an increase in bladder wall mass, muscle bundle size, and a threefold increase in the cross-sectional area of detrusor myocytes following PBOO in male New
Julie L Boerner et al.
Oncogene, 22(43), 6679-6689 (2003-10-14)
Mutations within members of the EGF/ErbB receptor family frequently release the oncogenic potential of these receptors, resulting in the activation of downstream signaling events independent of ligand regulatory constraints. We previously have demonstrated that the signal transduction events originating from
A Draeger et al.
FEBS letters, 291(1), 24-28 (1991-10-07)
Two-dimensional gel analysis of basic proteins in developing human smooth muscle identifies calponin as a prominent marker of the differentiated phenotype. Adult tissue (human and mouse) typically expresses up to four calponin isoforms, three of which appear sequentially during fetal
M J McManus et al.
Proceedings of the National Academy of Sciences of the United States of America, 94(21), 11351-11356 (1997-10-23)
The avian erythroblastosis viral oncogene (v-erbB) encodes a receptor tyrosine kinase that possesses sarcomagenic and leukemogenic potential. We have expressed transforming and nontransforming mutants of v-erbB in fibroblasts to detect transformation-associated signal transduction events. Coimmunoprecipitation and affinity chromatography have been
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