生物来源
rabbit
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen 130 kDa
种属反应性
mouse, human, rat
技术
immunoprecipitation (IP): 3 μg using using Protein A-agarose and 10 μg lysate of cultured ECV304 human endothelial cells or mouse fibroblasts NIH3T3 cell lysate
microarray: suitable
western blot: 1:1,000 using whole extract of the human ECV304 endothelial cells or a whole extract of the rat skeletal muscle myoblasts L8 cell line
UniProt登记号
储存温度
−20°C
基因信息
human ... BCAR1(9564)
mouse ... Bcar1(12927)
rat ... Bcar1(25414)
一般描述
p130cas or CAS (Crk-associated substrate) is a 120-130kD docking protein consists of a repetitive stretch of tyrosine-containing motifs. It has a pivotal role in cell adhesion, cell migration, cytokine receptor engagement and growth factor stimulation. It also has a critical role in cell transformation. Anti-(p130cas) antibody can be used in western blotting (diluted 1:1000) using human ECV304 endothelial cell extracts and rat skeletal muscle myoblasts L8 cell line extract. Rabbit anti-(p130cas) antibody reacts specifically with p130cas of rat, mouse and human.
免疫原
Synthetic peptide corresponding to the C-terminal region of human (p130cas) (amino acids 642-659), coupled to KLH. This sequence is identical in mouse and rat (p130cas) and has no homology to Cas-related proteins Hef1/Cas-L and Efs/Sin.
应用
Anti-(p130cas) antibody can be used in immunoprecipitation and microarray.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
未找到合适的产品？
试试我们的产品选型工具.
法规信息
新产品
此项目有
Differential regulation of cell migration, actin stress fiber organization, and cell transformation by functional domains of Crk-associated substrate.
Huang J, Hamasaki H
Biological Chemistry, 277(30), 27265-27272 (2002)
Fariborz Nasertorabi et al.
Biochemical and biophysical research communications, 324(3), 993-998 (2004-10-16)
The docking protein p130Cas becomes phosphorylated upon cell adhesion to extracellular matrix proteins, and is thought to play an essential role in cell transformation. Cas transmits signals through interactions with the Src-homology 3 (SH3) and Src-homology 2 domains of FAK
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持