Anti-m-Calpain (Domain III), Large Subunit antibody produced in rabbit

synthetic peptide corresponding to the N-terminal of the domain-III in the large subunit of rat m-calpain (E.C. 3.4.22.17, calpain-II, protein kinase-C activating factor).

Anti-m-Calpain (Domain III), Large Subunit antibody produced in rabbit is suitable for western blotting at a working dilution of 1:1,000 using an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as substrate.

By immunoblotting against the reduced protein, the antibody reacts with bands at 80 kDa and 58 kDa and a series of further cleaved active forms. It also reacts with non-reduced m-calpain. The antibody does not cross-react with other calpain family members (μ-calpain, calpain-94, ncl-2, ncl-3, etc.).

The calpains are calcium-activated non-lysosomal thiolproteases. m-Calpain is called as calpain-II. It is an intracellular, Ca2+-dependent cysteine protease. m- and μ-calpain are ubiquitously expressed and are countered by the endogenous calpain inhibitor calpastatin. The calpain family members are heterodimers. Calpains are present in all mammalian tissues and are involved in a variety of processes including cell proliferation, differentiation, vesicle secretion and others.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 50% glycerol, 1% BSA and 0.02% sodium azide

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