biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 38 kDa
species reactivity
mouse, human
technique(s)
immunoprecipitation (IP): 5 μg using 20 μg of a human A431 cell lysate, microarray: suitable, western blot: 1:1,000-1:5,000 using using human A431 and human Raji cell lysate, respectively
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... CRKL(1399)
mouse ... Crkl(12929)
General description
CrkL is a 39kD adaptor protein plays a crucial role in the early steps of IL-2 signal transduction. It also facilitates signaling of β-integrin, B-cell or T-cell receptors. Anti-Crk-L antibody can be used in immunoprecipitation for detecting and localizing CrkL. Rabbit anti-Crk-L antibody reacts specifically with CrkL (38 kD) of human.
Immunogen
A synthetic peptide corresponding to amino acids 283-303 of human Crk-L, conjugated to KLH. The sequence is highly conserved in mouse Crk-L (single amino acid substitution).
Application
Anti-Crk-L antibody can be used in western blotting (diluted 1: 1000, 1:5000) using human epidermal carcinoma A431 cell line and Burkitt lymphoma Raji cell line.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
F Gesbert et al.
The Journal of biological chemistry, 273(7), 3986-3993 (1998-03-28)
Interleukin (IL)-2, a major growth and differentiation factor for T lymphocytes, was found to induce tyrosine phosphorylation of the proto-oncogene products p120-Cbl and CrkL in IL-2-dependent cell lines. We established that, in unstimulated lymphocytes, the Src homology 2 (SH2) and
M Sattler et al.
Leukemia, 12(5), 637-644 (1998-05-21)
CRKL is a 39 kDa adapter protein, originally cloned in proximity to the BCR gene on chromosome 22, which has a key regulatory role in hematopoietic cells. CRKL has one SH2 and two SH3 domains, with 60% homology to CRK
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