表单
powder
技术
cell culture | mammalian: suitable
表面覆盖度
6‑10 μg/cm2
溶解性
aqueous acid: soluble (1 - 2μl acetic acid per ml H2O)
储存温度
2-8°C
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一般描述
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
应用
This collagen has been tested in culture with mammalian cells to verify it is low in endotoxin content. Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues. It is often used in cell culture as an attachment substratum. Myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have been cultured successfully on films or gels of Type I collagen. Type I collagen differs from other collagens by their low lysine hydroxylation and low carbohydrate composition.
外形
Lyophilized from 10 mM glacial acetic acid
制备说明
Prepared by a modification of the pepsin extraction method of Niyibizi, et al., J. Biol. Chem., 259, 14170 (1984).
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
C Niyibizi et al.
The Journal of biological chemistry, 259(22), 14170-14174 (1984-11-25)
Human type V collagen was purified from placenta and found to contain alpha 1(V), alpha 2(V), and alpha 3(V) chains in varying ratios. Using any of three independent nondenaturing methods (phosphocellulose chromatography, high-performance ion-exchange chromatography on IEX-540 DEAE, and ammonium
Akiko Nagaishi et al.
Nihon rinsho. Japanese journal of clinical medicine, 71(5), 876-880 (2013-06-20)
Patients with myasthenia gravis(MG) are divided into three groups: (1) acetylcholine receptor antibody positive MG: 80%, (2) muscle-specific receptor tyrosine kinase (MuSK) antibody positive MG: 5-10%, and (3) double seronegative MG. In 2011, autoantibodies (Abs) against low-density lipoprotein receptor-related protein
Katarina Wolf et al.
The Journal of cell biology, 201(7), 1069-1084 (2013-06-27)
Cell migration through 3D tissue depends on a physicochemical balance between cell deformability and physical tissue constraints. Migration rates are further governed by the capacity to degrade ECM by proteolytic enzymes, particularly matrix metalloproteinases (MMPs), and integrin- and actomyosin-mediated mechanocoupling.
V K Il'in et al.
Vestnik Rossiiskoi akademii meditsinskikh nauk, (2)(2), 56-62 (2013-07-04)
Dysbiotic shifts in intestinal and pharyngeal microflora were studied in 22 normal volunteers in 9-, 14-, 105- and 520-d chamber experiments simulating some of the spaceflight factors. Two preparations were administered to prevent pharyngeal and intestinal dysbiosis: oral dry probiotic
Visian toric ICL implantation for residual refractive errors after ICRS implantation and corneal collagen cross-linking in keratoconus.
Elias Jarade et al.
Journal of refractive surgery (Thorofare, N.J. : 1995), 29(7), 444-444 (2013-07-04)
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