血浆铜蓝蛋白 牛

血浆铜蓝蛋白 (Cp) 是血浆中主要的铜载体，被认为与脂蛋白的氧化修饰有关，并参与急性期应激反应。Cp 是一种可用于多种自由基的有效抗氧化剂，并且在还原型谷胱甘肽存在的条件下可以分解过氧化氢，表现出强过氧化物酶活性。它以与浓度相关的方式抑制绿过氧物酶的过氧化物酶活性，并且在体外显示出与绿过氧物酶选择性结合的性质。这些都说明了血浆铜蓝蛋白可能参与体内绿过氧物酶的清除和灭活。在生理学浓度（微摩尔级）下，发现它可以抑制由内皮依赖性激动剂（如乙酰胆碱或 ADP）引起的兔主动脉舒张。然而，研究发现它对由于硝酸甘油对平滑肌细胞直接刺激而引起的血管舒张无效。

Ceruloplasmin (Cp), the principal carrier of copper in plasma, is thought to be involved in oxidative modification of lipoproteins and participate in the acute phase reaction to stress. Cp is an effective antioxidant for a variety of radicals and has a potent peroxidase activity to decompose hydrogen peroxide in the presence of reduced glutathione. It inhibits the peroxidase activity of myeloperoxidase in a concentration-dependent manner and shows selective binding to myeloperoxidase in vitro. This suggests that ceruloplasmin may participate in the clearance and inactivation of myeloperoxidase in vivo. At physiological (micromolar) concentrations it was found to inhibit relaxation of rabbit aorta induced by endothelium-dependent agonists like acetylcholine or ADP. However, it was found ineffective toward vasodilation due to direct stimulation of smooth muscle cells by nitroglycerin. Cp plays an essential role in iron homeostasis, regulating the efflux of iron from macrophages and the oxidation of Fe²⁺ to Fe³⁺. Cp deficiency leads to neurodegenerative disorders, possibly by starving neurons of iron.

0.25M 氯化钠、0.05M 醋酸钠溶液，pH ~7.0

由缩二脲测定的蛋白质

在 pH 6.4 和 37°C 下以 N,N-二甲基对苯二胺为底物，一单位本品于 7mL 反应体积中每分钟产生的 ΔA₅₅₀ 为 0.01。