C2497
Anti-CPSF1 (C-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-CPSF160, Anti-Cleavage and polyadenylation specific factor 1, 160kDa, Anti-HSU37012, Anti-P/cl.18
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~160 kDa
species reactivity
human
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 5-10 μg using lysate of HEK-293T cells over expressing human CPSF1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... CPSF1(29894)
mouse ... Cpsf1(94230)
rat ... Cpsf1(366952)
Application
Anti-CPSF1 (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a working amount of 5-10μg using lysate of HEK-293T cells over expressing human CPSF1.
Biochem/physiol Actions
Cleavage and polyadenylation specificity factor subunit 1 is a protein encoded by the CPSF1 gene in humans. CPSF1 is the largest subunit of the CPSF complex composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1 and is located in the nucleus. CPSF is a multi-subunit protein that plays a central role in 3′ processing of mammalian pre-mRNAs. CPF interacts directly with the AAUAAA recognition signal in the RNA and is required for the cleavage as well as for the subsequent polyadenylation reaction during 3′ processing of messenger RNA precursors.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Qin Yang et al.
Wiley interdisciplinary reviews. RNA, 2(5), 732-747 (2011-08-09)
3' processing is an essential step in the maturation of all messenger RNAs (mRNAs) and is a tightly coupled two-step reaction: endonucleolytic cleavage at the poly(A) site is followed by the addition of a poly(A) tail, except for metazoan histone
W Keller et al.
The EMBO journal, 10(13), 4241-4249 (1991-12-01)
Cleavage and polyadenylation factor (CPF) is required for the cleavage as well as for the subsequent polyadenylation reaction during 3' processing of messenger RNA precursors. Here, we have investigated the interaction of CPF and poly(A) polymerase with short RNA substrates.
K G Murthy et al.
Genes & development, 9(21), 2672-2683 (1995-11-01)
Cleavage-polyadenylation specificity factor (CPSF) is a multisubunit protein that plays a central role in 3' processing of mammalian pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis.
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