biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen (caspase 8a) 55 kDa, antigen (caspase 8b) 54 kDa
species reactivity
human
technique(s)
microarray: suitable, western blot: 1:1,000 using human Burkitt lymphoma Raji cell extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... CASP8(841)
Immunogen
synthetic peptide of the human caspase 8a/MACHα1 precursor (amino acids 362-374 with N-terminal added lysine), conjugated to KLH.
Application
Anti-Caspase 8 (GD-13) is suitable for microarray. It is also suitable for western blot at a concentration of 1:1,000 using human Burkitt lymphoma Raji cell extract.
Biochem/physiol Actions
Caspase 8 cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. It also cleaves ADPRT. Caspase 8 hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. It is also a target for cowpox virus CRMA death inhibitory protein. Anti-Caspase 8 (GD-13) recognizes human caspase 8a (55 kDa) and caspase 8b (54kDa) by immunoblotting. Staining of caspase 8 in immunoblotting is specifically inhibited with caspase 8 immunizing peptide (human, amino acids 362-374). Caspases play a crucial role in apoptosis in almost every cell type.
The sequence used as the immunogen corresponds to the C-terminal region of the enzyme p18 subunit and is identical in other human caspase 8 isoforms (MACHα2, MACHα3, MACHβ) and has limited homology (approx. 50%) to mouse caspase 8. By immunoblotting, the antibody reacts with caspase 8a and caspase 8b.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
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