Cleaves the substrates consisting of consensus sequence DEVD.

Member of the CED-3 subfamily, the effector caspases, with an essential role in the apoptotic process. Upon activation, the zymogen is translocated to the mitochondrial and microsomal fractions. It is responsible for the cleavage of cellular substrates that precipitate the morphological changes of apoptosis.

Member of the CED-3 subfamily, the effector caspases; upon activation, the zymogen is translocated to the mitochondrial and microsomal fractions.

Useful in screening caspase inhibitors, studying enzyme regulation, determining specificities of caspase substrates, as well as using as a positive control in caspase activity assays and Western blot analyses.

Solution in HEPES, NaCl, DTT and sucrose, without carrier protein

Migrates as two major polypeptides (20 kDa and 10 kDa) in immunoblotting. Two minor polypeptides derived from the large subunit are also present.

One unit will hydrolyze 1.0 nmol of Ac-Asp-Glu-Val-Asp-AFC per minute at pH 7.5 at 25 °C.