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Merck
CN

C3028

Sigma-Aldrich

辅脂酶 来源于猪胰腺

essentially salt-free, lyophilized powder

别名:

辅脂酶 来源于猪胰腺

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关于此项目

CAS Number:
55126-92-6
EC 号:
259-490-1
MDL编号:
MFCD00130823
UNSPSC代码:
12352204
NACRES:
NA.54

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生物来源

Porcine pancreas

质量水平

200

方案

≥95% (Lowry)

表单

essentially salt-free, lyophilized powder

浓度

≥500 μ protein/vial

颜色

white to faint yellow

溶解性

H2O: soluble 1.9-2.1 mg/mL, clear, colorless to faintly yellow

适用性

suitable for molecular biology

UniProt登记号

P02703

应用

food and beverages

储存温度

−20°C

基因信息

pig ... CLPS(397407)

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相关类别

一般描述

基因脂肪酶（CLPS）编码一种主要在外分泌胰腺中表达的脂肪酶。这种蛋白质也存在于胃肠道中。它的结构包括17个氨基酸信号肽和一个位于N端的5氨基酸前体片段。
研究领域：细胞信号传导
辅脂肪酶是一种富含半胱氨酸的小分子蛋白，二级结构不多。结构呈扁平状，大小25×30×35 Å，总体类似一组突出的手指，在一端通过5个二硫键桥形成的复杂网络相互连接。辅脂肪酶结构包含两个拓扑性质十分相近的小结构域。

应用

来自猪胰腺的大肠酶已用于：

  • 脂肪酶测定的酶液
  • 在酶混合物中模拟肠胃消化
  • 作为明显关键因素之一，研究对番茄红素体外可及性的影响

生化/生理作用

脂质酶参与脂质代谢和凋亡信号通路。它对脂肪消化至关重要。作为一种胰腺蛋白，它可以防止脂肪酶变性，并能够附着在液滴的脂-水间相。脂质酶使脂肪酶可接近三酰甘油的内芯。外分泌胰腺功能不足引起的脂质酶分泌缺陷使膳食脂肪的腔内水解降至最低水平。缺乏先天性脂肪酶或脂质酶导致胰腺脂肪吸收不良。
辅脂肪酶(Colipase)，是一种蛋白辅因子，对胰脂肪酶进行的高效膳食脂质水解有重要作用。它连接脂肪酶的非催化性C末端结构域，稳定活性构象，显著增强整体的疏水结合位点。这种胰外分泌蛋白还帮助胰甘油三酯脂酶(PTL)吸附底物的脂水界面。

特点和优势

  • 克服胆汁盐对脂肪酶的抑制作用。
  • 可以在三油精、脱氧胆酸钠、以及猪胰脂肪酶的乳浊液中通过比浊法对活性进行确定。

制备说明

以1.9-2.1 mg/mL浓度溶于水，形成透明、无色至淡黄色溶液。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000376875
0000328495
0000328495
0000376875
0000240031

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the molecular weight of Product C3028, Colipase from porcine pancreas?

    According to Biochim. Biophys. Acta, 271, 400 (1972) the molecular weight of this protein is approximately 10,000 daltons, based on analysis by several techniques (diffusion sedimentation, and gel filtration). A molecular weight of 10,325 daltons has been calculated based on the amino acid composition and amino acid sequence of colipase, which is described in the Journal of Lipid Research, 20, 805-816, (1979).This product is likely to be a mixture of Colipase I and II as described in the following reference: Erlanson, C., et al., Biochim. Biophys. Acta, 310, 437, (1973). This is based on our SDS-PAGE results. The gel pattern shows two major bands, which probably correspond to the two forms of colipases. Minor bands at higher molecular weight may be pro-colipases, or perhaps other unrelated porcine pancreatic proteins.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Pancreatic colipase: chemistry and physiology.
B Borgström et al.
Journal of lipid research, 20(7), 805-816 (1979-09-01)
Colipase Stabilizes the Lid Domain of Pancreatic Triglyceride Lipase ast
Lowe, Mark E.
The Journal of Biological Chemistry, 272, 9-12 (1997)
W Junge et al.
Clinica chimica acta; international journal of clinical chemistry, 123(3), 293-302 (1982-08-18)
Colipase, like other pancreatic proteins, is liberated into the circulation in acute pancreatitis. Its concentration was measured in serum by a turbidimetric and in urine by a titrimetric method. The principle of both assays is based on the reactivation of
H van Tilbeurgh et al.
Biochimica et biophysica acta, 1441(2-3), 173-184 (1999-11-26)
Colipase is a small protein cofactor needed by pancreatic lipase for the efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilising an active conformation and considerably increasing the overall hydrophobic binding site. Structural studies
M D Yago et al.
The British journal of nutrition, 78(1), 27-39 (1997-07-01)
The aim of the present study was to investigate in human subjects whether or not the ingestion of two liquid meals that differed only in their fatty acid composition (due to the addition of olive oil (group O) or sunflowerseed
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