储存温度
−20°C
一般描述
This product consists of primer pairs specific for a region of the Arabidopsis ubiquitin-conjugating enzyme gene (UBC, At5g25760). It is intended for use as an internal control in RT-PCR analysis of Arabidopsis gene expression. Please see the Product Bulletin for more information on the use of this primer set.
应用
Primer should be stored at -20 °C. Primers are stable at -20 °C for at least 1 year and can be frozen and thawed at least 10 times.
其他说明
Each tube contains enough primer (forward or reverse) for 100 RT-PCR reactions (50 mL reaction volume)
- Forward Primer (Tm = 62 °C based on % GC method in 1 M salt concentration), 20 mM, in TE buffer
- Reverse Primer (Tm = 64 °C based on % GC method in 1 M salt concentration), 20 mM, in TE buffer
储存分类代码
12 - Non Combustible Liquids
法规信息
新产品
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Tomasz Czechowski et al.
Plant physiology, 139(1), 5-17 (2005-09-17)
Gene transcripts with invariant abundance during development and in the face of environmental stimuli are essential reference points for accurate gene expression analyses, such as RNA gel-blot analysis or quantitative reverse transcription-polymerase chain reaction (PCR). An exceptionally large set of
Matthäus Felsenstein et al.
Laboratory investigation; a journal of technical methods and pathology, 100(7), 1003-1013 (2020-02-02)
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