C3374
Cytochrome P450 Microsome Preparation human
3A4 Isozyme, recombinant, expressed in Saccharomyces cerevisiae, coexpressed with human NADPD reductase and human cytochrome b5
别名:
Cerosomes
concentration
15-25 mg protein/mL (Bradford assay)
biological source
human
recombinant
expressed in Saccharomyces cerevisiae
form
solution
specific activity
1.5-2.0 units/pmol enzyme
secondary activity
1300-1700 units/mg protein (Reductase activity)
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CYP3A4(1576)
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Physical form
Supplied as a solution containing 50 mM potassium phosphate, pH 7.4, 5% glycerol, 1 mM EDTA, 1 mM PMSF, and 2 mM DTT
Analysis Note
CYP450 content: 40.0-60.0 picomoles per mg protein
Other Notes
For more more information visit our drug metabolism resources for Cytochrome P450 microsome preparations.
P450 Activity: One unit will oxidize 1.0 picomole of 7-benzoxymethyloxy-3-cyanocoumarin per minute at pH 7.4 at 37 °C.
Reductase Activity: One unit will reduce 1 nanomole of cytochrome c per minute in the presence of NADPH at pH 7.8 at 25 °C.
Reductase Activity: One unit will reduce 1 nanomole of cytochrome c per minute in the presence of NADPH at pH 7.8 at 25 °C.
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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