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Merck
CN

C3667

软骨素酶 ABC 来源于普通变形杆菌

BSA free, lyophilized powder, specific activity 50-250 units/mg protein

别名:

4.2.2.20, 4.2.2.21, Chondroitin ABC Lyase

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关于此项目

化学文摘社编号:
9024-13-9
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-777-9
MDL number:
MFCD00130795
EC 号:
4.2.2.4 (BRENDA, IUBMB)

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产品名称

软骨素酶 ABC 来源于普通变形杆菌, BSA free, lyophilized powder, specific activity 50-250 units/mg protein

biological source

bacterial (Proteus vulgaris)

conjugate

(Glucosaminoglycan)

form

lyophilized powder

specific activity

50-250 units/mg protein

mol wt

120 kDa by gel filtration

composition

Protein, ~10% Lowry

solubility

0.01% bovine serum albumin aqueous (BSA) solution: soluble

application(s)

diagnostic assay manufacturing

foreign activity

protease, essentially free

storage temp.

−20°C

Quality Level

300

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相关类别

Application

该酶已被用于研究采用成年大鼠脑细胞，硫酸软骨素蛋白多糖所诱导的发急性、长期变化的后果。以新鲜牛角膜基质为原料，采用软骨素酶ABC对氨基聚糖进行酶解，测定了其在角质细胞培养中的含量。

Biochem/physiol Actions

硫酸软骨素酶ABC催化含有(1-4)-β-D-己糖胺基和(1-3)-β-葡聚糖或(1-3)-α-葡聚糖-L-iduronosyl与含有4-脱氧-β-D-葡聚糖-4-enuronosyl基团的双糖链接的清除性降解。它作用于4-硫酸软骨素、6-硫酸软骨素和皮肤硫酸软骨素，缓慢作用于透明质酸盐。分子量约为120 kDa，含有2个不相同的亚基，分子量分别为86 kDa和32 kDa。找到的最佳pH值与硫酸软骨素为8.0，与透明质酸为6.8，以及最佳温度是37°C。以1 mM Zn2+ 为抑制剂，0.05 M醋酸盐为酶的激活剂。

Other Notes

包含磷酸盐缓冲液和稳定剂
在37°C、pH 8.0条件下，一单位每分钟将释放1.0 μmole的2-乙酰氨基-2-脱氧-3-O-(β-D-葡萄糖)-4-烯-吡喃糖醛糖醛酸）-4-O-磺基-D-半乳糖的混合物和1.0 μmole来自鲨鱼软骨硫酸软骨素的2-乙酰氨基-2-脱氧-3-O-(β-D-葡萄糖-4-烯-吡喃糖醛糖醛酸)-6-O-磺基-D-半乳糖。
有关复合碳水化合物分析酶的更多信息，请见www.sigma-aldrich.com/enzymeexplorer

Packaging

基于硫酸软骨素 C活性包装。

Preparation Note

亲和纯化

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000442821
0000439605
0000442821
0000439605
0000198322

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Elizabeth J Bradbury et al.
Nature, 416(6881), 636-640 (2002-04-12)
The inability of axons to regenerate after a spinal cord injury in the adult mammalian central nervous system (CNS) can lead to permanent paralysis. At sites of CNS injury, a glial scar develops, containing extracellular matrix molecules including chondroitin sulphate
G Brückner et al.
Experimental brain research, 121(3), 300-310 (1998-09-24)
Lattice-like perineuronal accumulations of extracellular-matrix proteoglycans have been shown to develop during postnatal maturation and to persist throughout life as perineuronal nets (PNs) in many brain regions. However, the dynamics of their reorganization in adults are as yet unknown. The
Rong-Rong Zhao et al.
Neuroscience bulletin, 29(4), 477-483 (2013-07-11)
After spinal cord injury (SCI), re-establishing functional circuitry in the damaged central nervous system (CNS) faces multiple challenges including lost tissue volume, insufficient intrinsic growth capacity of adult neurons, and the inhibitory environment in the damaged CNS. Several treatment strategies
P N Bansal et al.
Osteoarthritis and cartilage, 18(2), 184-191 (2009-10-10)
An early hallmark of osteoarthritis (OA) is the progressive loss of glycosaminoglycans (GAGs), the extracellular matrix (ECM) component of articular cartilage that confers it with compressive stiffness. Our aim in this work is to establish the feasibility of using Contrast
Elizabeth J Bradbury et al.
Brain research bulletin, 84(4-5), 306-316 (2010-07-14)
Chondroitin sulphate proteoglycans (CSPGs) are potent inhibitors of growth in the adult CNS. Use of the enzyme chondroitinase ABC (ChABC) as a strategy to reduce CSPG inhibition in experimental models of spinal cord injury has led to observations of a
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