生物来源
human pancreas
质量水平
表单
solution
比活
50-55 units/mg protein carboxypeptidase B
杂质
≤0.2% chymotrypsin
≤0.2% trypsin
≤1 unit/mg protein carboxypeptidase A
UniProt登记号
运输
dry ice
储存温度
−20°C
SMILES字符串
S(Cc3ccccc3)C(=O)C(NC(=O)C2N(CCC2)C(=O)C(NC(=O)C(NC(=O)CCC(=O)O)C)C)Cc1ccccc1
InChI
1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
InChI key
TWURVFFNODFJBJ-UHFFFAOYSA-N
基因信息
human ... CPB1(1360)
一般描述
羧肽酶B定位于人类染色体3q24上。 羧肽酶B属于羧肽酶的A/B亚家族。
应用
来自Sigma的羧肽酶B作为参考标准品，已被用作测定源自大鼠前叶和中叶的裂解垂体颗粒中羧肽酶活性。 该酶还已被用于通过去除C末端碱性氨基酸对血浆样品进行消化，从而在C4电泳过程中为每种同种异型片段获得独特的条带。
生化/生理作用
羧肽酶B（CPB1）基因的突变与胰腺癌发生和发展的敏感性增加有关。 CPB1水平升高与低度乳腺肿瘤和淋巴结阳性1级肿瘤相关。
羧肽酶B（或肽基-L-赖氨酸（-L-精氨酸）水解酶）可催化碱性氨基酸，赖氨酸、精氨酸和鸟氨酸从多肽的C端水解。研究显示其为34,000Da的单链多肽。胰蛋白酶能够在体外将天然酶转化为活性酶羧肽酶B II。 其最适pH为9.0。该酶可通过连续切割C端碱性氨基酸从而用于序列分析。它也可以用作诊断急性胰腺炎的血清标志物。
外形
溶于0.05 M NaOAc（pH 5.0）、1.0 M NaCl、0.01％NaN3中的溶液
其他说明
在pH7.7，25℃条件下，一个单位每分钟可水解1 μmole马尿酰-L-精氨酸
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
此项目有
Xiu Qin Xu et al.
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The Biochemical journal, 239(3), 763-767 (1986-11-01)
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Journal of Molecular Biology, 321(3), 537-547 (2002)
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Journal of chromatography. A, 1216(32), 5989-5994 (2009-06-30)
A robust cation-exchange chromatofocusing method was developed for the routine analysis of a recombinant humanized monoclonal IgG antibody. We compare the chromatofocusing method to the conventional cation-exchange chromatography (CEX) employing a salt gradient and show clear advantages of chromatofocusing over
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