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Merck
CN

C5921

Sigma-Aldrich

Cholesterol Esterase from bovine pancreas

lyophilized powder, ≥10,000 units/g protein

别名:

Sterol-ester acylhydrolase

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表单

lyophilized powder

比活

≥10,000 units/g protein

储存温度

−20°C

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应用

Enzyme responsible for the hydrolysis of many of the fatty acid esters of cholesterol.
Optimum pH range: 6-8
Activators: Bisphenol A diglycidyl ether, cAMP-dependent protein kinase type II, ethanol, methanol, n-butanol, phosphatidylcholine, phosphatidylethanolamine, sodium taurocholic acid
Inhibitors: Bisphenol A methacrylate, diisopropylfluorophosphate, enolase, Hg2+, sodium fluoride, phosphatidic acid, phosphatidylcholine, phosphatidylserine

分析说明

Protein determined by biuret.

其他说明

One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

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历史批次信息供参考:

分析证书(COA)

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Maria J Pereira et al.
Molecular and cellular endocrinology, 365(2), 260-269 (2012-11-20)
Cyclosporin A (CsA), tacrolimus and rapamycin are immunosuppressive agents (IAs) associated with insulin resistance and dyslipidemia, although their molecular effects on lipid metabolism in adipose tissue are unknown. We explored IAs effects on lipolysis, lipid storage and expression of genes
Myung Hee Moon et al.
Experimental & molecular medicine, 44(10), 603-614 (2012-08-04)
Higher levels of body fat are associated with an increased risk for development numerous adverse health conditions. FTY720 is an immune modulator and a synthetic analogue of sphingosine 1-phosphate (S1P), activated S1P receptors and is effective in experimental models of
Joanna Karbowska et al.
Steroids, 77(13), 1359-1365 (2012-09-07)
Dehydroepiandrosterone (DHEA) reduces body fat in rodents and humans, and increases glycerol release from isolated rat epididymal adipocytes and human visceral adipose tissue explants. It suggests that DHEA stimulates triglyceride hydrolysis in adipose tissue; however, the mechanisms underlying this action
Yong-lan Huang et al.
Zhonghua er ke za zhi = Chinese journal of pediatrics, 50(8), 601-605 (2012-11-20)
To explore the clinical characteristics of Wolman disease and diagnostic methods using enzymatic and molecular analysis. Lysosomal acid lipase activity was measured using 4-methylumbelliferyl oleate in the leukocytes of an infant suspected of Wolman disease and LIPA gene mutational analysis
Vincent Delorme et al.
PloS one, 7(9), e46493-e46493 (2012-10-03)
Lipid metabolism plays an important role during the lifetime of Mycobacterium tuberculosis, the causative agent of tuberculosis. Although M. tuberculosis possesses numerous lipolytic enzymes, very few have been characterized yet at a biochemical/pharmacological level. This study was devoted to the

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