biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
clone
polyclonal
form
buffered aqueous glycerol solution
species reactivity
human, mouse, rat
concentration
~1 mg/mL
technique(s)
immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot (chemiluminescent): 1:5,000, western blot: 1:1,000
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... CAPN3(825)
mouse ... Capn3(12335)
rat ... Capn3(29155)
Immunogen
synthetic peptide corresponding to the C-terminal end of domain-III of the large subunit of human m-calpain (calpain-II, protein kinase C activating factor, E.C. 3.4.22.17).
Biochem/physiol Actions
Recognizes latent and active m-calpain. Binds the reduced and non-reduced protein. Identifies bands at 80 kDa, 58 kDa, and a series of further cleaved active forms.
Physical form
Solution in 0.01 M phosphate buffered saline, containing 50% glycerol and 0.05% sodium azide.
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