产品名称
单克隆抗-胶原蛋白，VII 型 小鼠抗, clone LH7.2, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
LH7.2, monoclonal
contains
15 mM sodium azide
species reactivity
human, goat, marmoset, monkey, guinea pig, sheep, pig, bovine
technique(s)
dot blot: suitable
immunocytochemistry: suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:1,000 using human or other mammalian frozen sections
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... COL7A1(1294)
Application
单克隆抗VII型胶原抗体可用作病毒滴定中的一抗。它也可以用在点免疫印迹、免疫细胞化学和 ELISA 等不同的免疫化学分析中定位 VII 型胶原蛋白。
小鼠单克隆抗-VII 型胶原蛋白抗体可用于免疫金标记。
Biochem/physiol Actions
VII 型胶原蛋白 α1 链 (COL7A1)是皮肤、粘膜和角膜上皮间质锚定所必需的。它被认为是转化生长因子 β（TGF-β）/SMAD（母亲 DPP 同源物）信号通路的早期反应基因。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
VII 型胶原蛋白 α1 链（COL7A1）主要在皮肤中表达，由角质形成细胞和成纤维细胞分泌。该基因位于人染色体3p21.31上。
胶原蛋白是一种纤维蛋白，存在于所有脊椎动物的细胞外骨架中。VII 型胶原蛋白是锚定原纤维的重要组成部分，VII 型胶原蛋白基因 (COL7A1) 突变会导致营养不良性大疱性表皮松解症。单克隆抗胶原 VII 型抗体可通过观察表皮基底膜的外观和完整性来区分侵袭性黑色素瘤和非侵袭性黑色素瘤。它还可用于免疫印迹法。小鼠抗 VII 型胶原蛋白抗体与位于胶原酶消化的 VII 型胶原蛋白 (150 kDa) 上的表位发生特异性反应。该产品还与绵羊、猪、牛、豚鼠、山羊和人的复层鳞状上皮的基底膜区域发生反应。
Immunogen
新生儿人包皮表皮细胞的不溶部分
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物来源生物产品
此项目有
Daisuke Sawamura et al.
The Journal of investigative dermatology, 118(6), 967-971 (2002-06-13)
The expression of intradermally injected DNA by keratinocytes is found mainly in the upper and middle layers of the epidermis. To investigate the mechanism of this selective expression, we observed the sequential changes in the distribution of interleukin-6-expressing keratinocytes after
Martin A Barbier et al.
Current protocols, 2(1), e353-e353 (2022-01-28)
Efficient gene transfer into cultured fibroblasts and keratinocytes during retroviral transduction is a critical step toward the treatment of genodermatoses such as epidermolysis bullosa. However, achieving high transduction rates is still a difficult task, particularly for the insertion of large
COL7A1 editing via CRISPR/Cas9 in recessive dystrophic epidermolysis bullosa
Hainzl S, et al.
Molecular Therapy, 25(11), 2573-2584 (2017)
Arkadii K Beilin et al.
International journal of molecular sciences, 22(4) (2021-03-07)
The recessive form of dystrophic epidermolysis bullosa (RDEB) is a debilitating disease caused by impairments in the junctions of the dermis and the basement membrane of the epidermis. Mutations in the COL7A1 gene induce multiple abnormalities, including chronic inflammation and
SMAD3/4-dependent transcriptional activation of the human type VII collagen gene (COL7A1) promoter by transforming growth factor ?
Vindevoghel L, et al.
Proceedings of the National Academy of Sciences of the USA, 95(25), 14769-14774 (1998)
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