Anti-phospho-c-Raf (pSer³³⁸/pTyr³⁴⁰) antibody produced in rabbit

c-Raf or Raf-1 belongs to the Raf family of kinases that have regulatory functions in cell growth, differentiation, growth factor receptor stimulation via the MAPK signalling. The other members of the family are A-Raf and B-Raf. All the isoforms have unique as well as overlapping regulatory functions. c-Raf kinase is the first identified downstream effector kinase of Ras, and is the activator of MEK which in turn activates ERK. Phosphorylation of c-Raf at Ser³³⁸ and Tyr³⁴⁰ regulates its biological function.
Anti-phospho-c-Raf [pSer³³⁸/pTyr³⁴⁰] detects human c-Raf [pSer³³⁸/pTyr³⁴⁰]hosphorylated at [pSer³³⁸/pTyr³⁴⁰]er338 and [pSer³³⁸/pTyr³⁴⁰]yr340 (74 kDa). Mouse and [pSer³³⁸/pTyr³⁴⁰]at c-Raf (100% homology) are [pSer³³⁸/pTyr³⁴⁰]xpected [pSer³³⁸/pTyr³⁴⁰]o cross react.

synthetic phosphopeptide derived from the region of human c-Raf that contains serine 338 and tyrosine 340.

The antibody may be used to detect phospho-c-Raf (Ser³³⁸/Tyr³⁴⁰) by immunoblotting at working dilution of 1:1000 in cell lysates from HEK293 cells transfected with c-Raf. Detection was also possible in spermatozoa of mice at 1:1000 dilution.

Solution in Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

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