recombinant
expressed in E. coli
assay
>90% (SDS-PAGE)
form
lyophilized powder
solubility
PBS: soluble
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CASP2(835), CASP3(836), CASP7(840)
Biochem/physiol Actions
Group II enzymes function in apoptosis as effectors and upstream activators and used in screening caspase inhibitors, studying enzyme kinetics and regulation, determining target substrates, as well as serving as positive controls in caspase activity assays and Western blot analysis.
Physical form
Contains 0.052% ammonium sulfate, 0.158% Tris−HCl, 0.76% sodium chloride.
Other Notes
One unit will hydrolyze 1 nmol of the caspase substrate VDVAD-pNA (for caspase-2) and DEVD-pNA (for caspase-3,7), respectively, per hour at pH 7.2 at 37 °C.
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