表单
lyophilized powder
比活
≥2.0 units/mg protein
分子量
27 kDa
组成
Protein, ≥75% E1%/280
溶解性
H2O: soluble 5.0 mg/mL, clear, colorless
储存温度
−20°C
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应用
Chymopapain from papaya latex has been used in a study to assess the anthelmintic effect of natural plant cysteine proteinases against the gastrointestinal nematode, Heligmosomoides. Chymopapain from papaya latex has also been used in a study to investigate the preparation and identification of the rabbit′s antibody of chymopapain.
The enzyme has been used to detach cultured CD34+ cells from beads (130 units/mL for 2 minutes, repeated three times). Chymopapain and pronase have been found to be effective in the digestion of human intestinal tissue. The enzyme has been used along with pronase, collagenase, elastase, DNAse, and catalase for rabbit lung cells digestion at a concentration of 0.05% in calcium-magnesium-free Kreb′s serum substitute. It has also been used for the release of neuroblastoma cells from marrow bound to antibody-coated microspheres.
生化/生理作用
Plant cysteine proteinase that is similar in function to lysosomal cathepsin L.
The enzyme cleaves amide linkages at the carboxyl side of arginine, leucine, glutamic acid, and alanine residues; as well as that of arginine esters. The optimum pH varies depending on the kind of substrate. For example, the pH optimum for casein digestion is 7.9, hemoglobin digestion is 4.0 and urea-denatured hemoglobin digestion is 7.0. Molecular mass of the enzyme is found to be 27 KDa.
制备说明
色谱纯化产品
其他说明
Contains L-cysteine and EDTA
One unit will hydrolyze 1.0 μmole of BAEE to Nα-benzoyl-L-arginine per min at pH 6.2 at 25 °C
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
靶器官
Respiratory system
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
新产品
此项目有
Silas P Rodrigues et al.
Journal of proteomics, 75(11), 3191-3198 (2012-04-03)
Papaya meleira virus (PMeV) is so far the only described laticifer-infecting virus, the causal agent of papaya (Carica papaya L.) sticky disease. The effects of PMeV on the laticifers' regulatory network were addressed here through the proteomic analysis of papaya
Sufang Wang et al.
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, 25(12), 891-893 (2003-04-11)
Chymopapain, one of the four cysteine proteinases of papaya latex, has milk clotting and proteolytic activity. It is mainly used to treat prolapsed intervertebral discs. This paper introduced the preparation of polyclonal antibody of partially purified chymopapain and the identification
Y Gazitt et al.
Cancer research, 52(10), 2957-2965 (1992-05-15)
A methodology for rapid isolation of neuroblastoma cells from marrow with metastatic neuroblastoma cells was developed using a cocktail of five antibodies and magnetic microspheres coated with secondary antibodies. Cells bound to microspheres were released by brief exposure to chymopapain
Dispersal of rabbit lung into individual viable cells: a new model for the study of lung metabolism.
K G Gould et al.
Science (New York, N.Y.), 178(4066), 1209-1210 (1972-12-15)
This new enzymatic method disperses rabbit lung into morphologically intact, viable individual cells. The scattered cells constitute more than 50 percent of the original tissue. At least 90 percent of the cells exclude trypan blue from the nucleus. The dispersed
C C Fox et al.
Journal of immunology (Baltimore, Md. : 1950), 135(1), 483-491 (1985-07-01)
Study of the role of mast cells in the human gastrointestinal tract has suffered from the inability to examine these cells in vitro. In addition, work in rodent systems suggests that there are substantial differences between intestinal mast cells and
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