C8618
Chondroitinase AC from Arthrobacter aurescens
aqueous glycerol solution
别名:
Chondroitin AC-Lyase
form
aqueous glycerol solution
specific activity
≥100 units/mg protein
shipped in
dry ice
storage temp.
−20°C
正在寻找类似产品? 访问 产品对比指南
Biochem/physiol Actions
Chondroitinase AC is an eliminase that degrades chondroitin sulfates A and C, but not chondroitin sulfate B. The enzyme cleaves, via an elimination mechanism, sulfated and non-sulfated polysaccharide chains containing (1-4) linkages between hexosamines and glucuronic acid residues. The reaction yields oligosaccharide products, mainly disaccharides, containing unsaturated uronic acids that can be detected by UV spectroscopy at 232 nm.
The enzyme shows approximately equal activity with chondroitin sulfates A and C, while the activity observed with chondroitin sulfate B is approximately 7% of this value. This activity is most likely due to the presence of chondroitin sulfates A and C (10%) in the chondroitin sulfate B.
The enzyme shows approximately equal activity with chondroitin sulfates A and C, while the activity observed with chondroitin sulfate B is approximately 7% of this value. This activity is most likely due to the presence of chondroitin sulfates A and C (10%) in the chondroitin sulfate B.
Physical form
Supplied as an aqueous solution in containing 0.02M Trizma-HCl pH 7.2 and 2% glycerol
Other Notes
One unit will liberate 1.0 μmole of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-4-O-sulfo-D-galactose from chondroitin sulfate A or 1.0 μmole of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-6-O-sulfo-D-galactose from chondroitin sulfate C per min at pH 6.0 at 37 °C.
ppe
Eyeshields, Gloves
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
K Pojasek et al.
Biochemical and biophysical research communications, 286(2), 343-351 (2001-08-14)
Glycosaminoglycans (GAGs) are a family of complex polysaccharides involved in a diversity of biological processes, ranging from cell signaling to blood coagulation. Chondroitin sulfate (CS) and dermatan sulfate (DS) comprise a biologically important subset of GAGs. Two of the important
Studies on the enzyme chondroitinase: product structure and ion effects.
H I NAKADA et al.
Archives of biochemistry and biophysics, 94, 244-251 (1961-08-01)
J Féthière et al.
Acta crystallographica. Section D, Biological crystallography, 54(Pt 2), 279-280 (1998-10-08)
Chondroitinase AC (E.C. 4.2.2.5) overexpressed in its host, Flavobacterium heparinum, was crystallized by vapor diffusion using polyethylene glycol methyl ether as precipitant. It crystallizes in the space group P43212 or its enantiomorph with a = b = 87.1 and c
Thomas N Huckerby et al.
The FEBS journal, 272(24), 6276-6286 (2005-12-13)
Chondroitin and dermatan sulfate (CS and DS) chains were isolated from bovine tracheal cartilage and pig intestinal mucosal preparations and fragmented by enzymatic methods. The oligosaccharides studied include a disaccharide and hexasaccharides from chondroitin ABC lyase digestion as well as
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持