biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 48 kDa
species reactivity
mouse, human
concentration
~1 mg/mL
technique(s)
immunoprecipitation (IP): 5-10 μg using extracts of K-562 cells., microarray: suitable, western blot: 0.5-1 μg/mL using extracts of K-562 cells, western blot: 1-2 μg/mL using mouse brain extracts
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... CTBP1(1487)
mouse ... Ctbp1(13016)
rat ... Ctbp1(29382)
General description
The gene CtBP1 (Carboxy terminal binding protein 1) is mapped to human chromosome 4p16.3. It belongs to the CtBP family of proteins. The protein localizes in the cytoplasm and the nucleus.
Immunogen
对应于人 CtBP1 的氨基酸 432-440 的合成肽。小鼠和大鼠相应的序列分别有一个和两个氨基酸的差异。此肽不与 CtBP2 发生交叉反应。
Application
Anti-CtBP1, C-Terminal antibody has been used as a primary antibody in western blot analysis of HIC1 (Hypermethylated in Cancer 1) proteins. It is also suitable for immunoblotting (48kDa), microarray and immunoprecipitation (5-10μg using extracts of K-562 cells).
Biochem/physiol Actions
Carboxy terminal binding protein 1 (CtBP1) is a NADH-dependent transcriptional repressor. It interacts with human polycomb protein (HPC2). It is able to bind with adenovirus type 2 and 5 E1A protein, and inhibits transcriptional activation and tumorigenesis mediated by the E1A protein. It is a co-repressor of BCL6 (B-cell lymphoma 6 protein) and Epstein-Barr virus oncoprotein EBNA3A. ZNF366 associates with CtBP1 to repress estrogen receptor-α (ERα) activity. Nuclear and cell adhesion-related phosphoprotein, pinin, binds with CtBP1 and releases the CtBP1-mediated repression of E-cadherin promoter activity. It represses expression of several tumor suppressor genes and regulates epithelial-mesenchymal transition during cancer cell metastasis.
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
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L Wang et al.
Cell death & disease, 6, e1620-e1620 (2015-01-31)
Cancer cells rely on glycolysis to maintain high levels of anabolism. However, the metabolism of glucose via glycolysis in cancer cells is frequently incomplete and results in the accumulation of acidic metabolites such as pyruvate and lactate. Thus, the cells
Xiao-Ling Zhang et al.
Oncology reports, 30(2), 809-814 (2013-06-13)
Hepatocellular carcinoma (HCC) is a highly invasive type of cancer. Metastasis is the leading cause of mortality of advanced HCC patients. In the metastasis cascade, cancer cells undergo epithelial-mesenchymal transition resulting in the loss of cell‑to‑cell adhesion, migration and invasion
Mark Hickabottom et al.
The Journal of biological chemistry, 277(49), 47197-47204 (2002-10-10)
CtBP (carboxyl-terminal binding protein) has been shown to be a highly conserved co-repressor of transcription that is important in development, cell cycle regulation, and transformation. Viral proteins E1A and EBNA3C and all the various Drosophila and vertebrate transcription factors to
Lai Man Natalie Wu et al.
Nature neuroscience, 19(8), 1060-1072 (2016-06-14)
The mechanisms that coordinate and balance a complex network of opposing regulators to control Schwann cell (SC) differentiation remain elusive. Here we demonstrate that zinc-finger E-box-binding homeobox 2 (Zeb2, also called Sip1) transcription factor is a critical intrinsic timer that
Balabhadrapatruni V S K Chakravarthi et al.
Molecular cancer research : MCR, 16(7), 1125-1137 (2017-10-14)
In aggressive prostate cancers, the oncoprotein STMN1 (also known as stathmin 1 and oncoprotein 18) is often overexpressed. STMN1 is involved in various cellular processes, including cell proliferation, motility, and tumor metastasis. Here, it was found that the expression of
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