C9268
羧肽酶 A 来源于牛胰腺
(Type II-PMSF treated), ≥50 units/mg protein, ready-to-use solution
别名:
羧肽酶, 肽基-L-氨基酸水解酶
等级
Proteomics Grade
表单
ready-to-use solution
质量
(Type II-PMSF treated)
比活
≥50 units/mg protein
分子量
~35 kDa
纯化方式
2× crystallization
杂质
≤0.05 BTEE units/mg protein chymotrypsin
≤10 BAEE units/mg protein trypsin
储存温度
2-8°C
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应用
来自牛胰腺的羧肽酶 A 被用于研究牛羧肽酶 A 的可溶和可活化形式在 大肠杆菌中的表达。来自牛胰腺的羧肽酶 A 也被用于研究鸵鸟羧肽酶前体的分离和部分特性。
用 Sigma 公司生产的羧肽酶研究了绿僵菌羧肽酶 A (MeCPA) 的特异性。MeCPA 是经过基因工程改造的，便于从重组蛋白的 C-末端去除多聚组氨酸标签。在体外还用于脱酪氨酸 α-微管蛋白，以诱导与乙基-N-苯氨基甲酸酯 (EPC) 琼脂糖的高亲和力。
生化/生理作用
羧肽酶分离自牛胰腺腺，是一种金属酶，每摩尔蛋白质含有 1 g 锌原子。对肽类和蛋白质中羧基末端肽键的水解进行催化。主要特异于芳香族和疏水侧链，如苯丙氨酸、色氨酸或亮氨酸。这种酶也表现出酯酶活性。可被 β-苯基丙酸盐和吲哚乙酸盐抑制。
制备说明
用苯甲基磺酰氟处理以消除胰蛋白酶和糜蛋白酶活性。透析和重结晶：加入甲苯的水性混悬液。
分析说明
通过 E 1%/278 测定蛋白
其他说明
25°C、pH 7.5 下 1 个单位每分钟水解 1.0 μmol 马尿酸-L-苯丙氨酸。
Bodo Wiesler et al.
The Plant journal : for cell and molecular biology, 32(6), 1023-1032 (2002-12-21)
Auxin controls the orientation of cortical microtubules in maize coleoptile segments. We used tyrosinylated alpha-tubulin as a marker to assess auxin-dependent changes in microtubule turnover. Auxin-induced tyrosinylated alpha-tubulin, correlated with an elevated sensitivity of growth to antimicrotubular compounds such as
Brian P Austin et al.
Protein expression and purification, 77(1), 53-61 (2010-11-16)
Carboxypeptidases may serve as tools for removal of C-terminal affinity tags. In the present study, we describe the expression and purification of an A-type carboxypeptidase from the fungal pathogen Metarhizium anisopliae (MeCPA) that has been genetically engineered to facilitate the
Zhenhua Li et al.
BMC bioinformatics, 13, 51-51 (2012-03-29)
Water is an integral part of protein complexes. It shapes protein binding sites by filling cavities and it bridges local contacts by hydrogen bonds. However, water molecules are usually not included in protein interface models in the past, and few
Laurence Lafanechère et al.
Methods in molecular biology (Clifton, N.J.), 777, 71-86 (2011-07-21)
Alpha tubulin comprises a C-terminal tyrosine residue, which is subject to cyclic removal from the peptide chain by a still uncharacterized carboxypeptidase and re-addition to the chain by a tubulin tyrosine ligase. We have shown in different animal or human
Heiko Witt et al.
Nature genetics, 45(10), 1216-1220 (2013-08-21)
Chronic pancreatitis is an inflammatory disorder of the pancreas. We analyzed CPA1, encoding carboxypeptidase A1, in subjects with nonalcoholic chronic pancreatitis (cases) and controls in a German discovery set and three replication sets. Functionally impaired variants were present in 29/944
实验方案
在测定羧肽酶A活性时，使用马尿酸-L-苯丙氨酸在254nm处进行连续分光光度法测定。羧肽酶A水解C末端残基上的肽键。
Carboxypeptidase A activity measured via continuous spectrophotometric rate determination assay with hippuryl-L-phenylalanine substrate.
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