C9499
耐高温纤维素酶 from Clostridium thermocellum
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
别名:
1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase
重组
expressed in E. coli
质量水平
方案
≥90% (SDS-PAGE)
表单
liquid
比活
≥40 units/mg protein
分子量
41 kDa
浓度
19-21 mg protein/mL (UV)
运输
wet ice
储存温度
2-8°C
应用
Cellulases are enzymes that hydrolyze cellulose to glucose. Cellulase is used to study the development of occupational asthma in the detergent, pharmaceutical, baking, and enzyme production industries . Cellulase is added to detergents to improve cleansing properties . Cellulase, product C9499, is from Clostridium thermocellum and is recombinant and expressed in E. coli.
生化/生理作用
Cellulases are produced by the intestinal flagellates of termites which are important for cellulose digestion of their host termites . Cellulases have different microbial origins and therefore different protein sequences . Product C9499 exhibits endo-cellulase, β-glucanase activity when assayed using insoluble AZCL-linked substrates as well as exo-cellulase and cellobiohydralase activities.
外形
Supplied as as solution in 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, and 25% glycerol.
其他说明
One unit will produce 1 μmole of reducing sugar (measured as glucose) from β-glucan per minute at pH 5.8 at 70 °C.
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Gaku Tokuda et al.
Biology letters, 3(3), 336-339 (2007-03-22)
The intestinal flagellates of termites produce cellulases that contribute to cellulose digestion of their host termites. However, 75% of all termite species do not harbour the cellulolytic flagellates; the endogenous cellulase secreted from the midgut tissue has been considered a
A Brant et al.
Occupational and environmental medicine, 61(9), 793-795 (2004-08-20)
Three employees from two different detergent companies were investigated for occupational asthma, using skin prick tests, serum specific IgE, and specific bronchial challenge. Two were challenged with lipase and one with cellulase. All three cases had immunological evidence of sensitisation
Raphaël Dutoit et al.
PloS one, 7(11), e50639-e50639 (2012-12-12)
Several aminopeptidases of the M42 family have been described as tetrahedral-shaped dodecameric (TET) aminopeptidases. A current hypothesis suggests that these enzymes are involved, along with the tricorn peptidase, in degrading peptides produced by the proteasome. Yet the M42 family remains
Qiuhong Niu et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 52(11), 1408-1414 (2013-02-07)
To screen, identify bacterial strains with high capability to degrade cellulose from bacteria associated with Bursaphelenchus xylophilus and to clone related genes. First, we collected B. xylophilus samples from pine wood nematode disease areas in Nanyang, Henan province, China. Then
Wenjie Gu et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 52(9), 1085-1093 (2012-12-15)
The aim of this study was to screen microorganisms that could degrade rice straw. We used selective medium to screen strains and determined straw fracture tension strength, weight loss, lignocellulose decomposition rate and extracellular enzyme activity as re-screening methods after
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