C9499
耐高温纤维素酶 from Clostridium thermocellum
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
别名:
1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase
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关于此项目
Specific activity:
≥40 units/mg protein
Assay:
≥90% (SDS-PAGE)
Recombinant:
expressed in E. coli
Concentration:
19-21 mg protein/mL (UV)
recombinant
expressed in E. coli
assay
≥90% (SDS-PAGE)
form
liquid
specific activity
≥40 units/mg protein
mol wt
41 kDa
concentration
19-21 mg protein/mL (UV)
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Application
Cellulases are enzymes that hydrolyze cellulose to glucose. Cellulase is used to study the development of occupational asthma in the detergent, pharmaceutical, baking, and enzyme production industries . Cellulase is added to detergents to improve cleansing properties . Cellulase, product C9499, is from Clostridium thermocellum and is recombinant and expressed in E. coli.
Biochem/physiol Actions
Cellulases are produced by the intestinal flagellates of termites which are important for cellulose digestion of their host termites . Cellulases have different microbial origins and therefore different protein sequences . Product C9499 exhibits endo-cellulase, β-glucanase activity when assayed using insoluble AZCL-linked substrates as well as exo-cellulase and cellobiohydralase activities.
Physical form
Supplied as as solution in 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, and 25% glycerol.
Other Notes
One unit will produce 1 μmole of reducing sugar (measured as glucose) from β-glucan per minute at pH 5.8 at 70 °C.
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Gaku Tokuda et al.
Biology letters, 3(3), 336-339 (2007-03-22)
The intestinal flagellates of termites produce cellulases that contribute to cellulose digestion of their host termites. However, 75% of all termite species do not harbour the cellulolytic flagellates; the endogenous cellulase secreted from the midgut tissue has been considered a
A Brant et al.
Occupational and environmental medicine, 61(9), 793-795 (2004-08-20)
Three employees from two different detergent companies were investigated for occupational asthma, using skin prick tests, serum specific IgE, and specific bronchial challenge. Two were challenged with lipase and one with cellulase. All three cases had immunological evidence of sensitisation
Qiuhong Niu et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 52(11), 1408-1414 (2013-02-07)
To screen, identify bacterial strains with high capability to degrade cellulose from bacteria associated with Bursaphelenchus xylophilus and to clone related genes. First, we collected B. xylophilus samples from pine wood nematode disease areas in Nanyang, Henan province, China. Then
Wenjie Gu et al.
Wei sheng wu xue bao = Acta microbiologica Sinica, 52(9), 1085-1093 (2012-12-15)
The aim of this study was to screen microorganisms that could degrade rice straw. We used selective medium to screen strains and determined straw fracture tension strength, weight loss, lignocellulose decomposition rate and extracellular enzyme activity as re-screening methods after
You-Shao Yan et al.
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, 35(7), 1144-1147 (2012-12-21)
To study the optimum extraction process of total flavonoids from Psoralea coryl folia by cellulose-assisted technique. Based on single-factor experiments, the effects of pH value, temperature of enzymatic hydrolysis, time of enzymatic hydrolysis and enzyme amount on the extraction yields
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