C9499
耐高温纤维素酶 from Clostridium thermocellum
recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
别名:
1,4-(1,3:1,4)-β-D-Glucan 4-glucano-hydrolase
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关于此项目
Specific activity:
≥40 units/mg protein
Assay:
≥90% (SDS-PAGE)
Recombinant:
expressed in E. coli
Concentration:
19-21 mg protein/mL (UV)
recombinant
expressed in E. coli
Quality Level
assay
≥90% (SDS-PAGE)
form
liquid
specific activity
≥40 units/mg protein
mol wt
41 kDa
concentration
19-21 mg protein/mL (UV)
shipped in
wet ice
storage temp.
2-8°C
Application
Cellulases are enzymes that hydrolyze cellulose to glucose. Cellulase is used to study the development of occupational asthma in the detergent, pharmaceutical, baking, and enzyme production industries . Cellulase is added to detergents to improve cleansing properties . Cellulase, product C9499, is from Clostridium thermocellum and is recombinant and expressed in E. coli.
Biochem/physiol Actions
Cellulases are produced by the intestinal flagellates of termites which are important for cellulose digestion of their host termites . Cellulases have different microbial origins and therefore different protein sequences . Product C9499 exhibits endo-cellulase, β-glucanase activity when assayed using insoluble AZCL-linked substrates as well as exo-cellulase and cellobiohydralase activities.
Physical form
Supplied as as solution in 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, and 25% glycerol.
Other Notes
One unit will produce 1 μmole of reducing sugar (measured as glucose) from β-glucan per minute at pH 5.8 at 70 °C.
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Gaku Tokuda et al.
Biology letters, 3(3), 336-339 (2007-03-22)
The intestinal flagellates of termites produce cellulases that contribute to cellulose digestion of their host termites. However, 75% of all termite species do not harbour the cellulolytic flagellates; the endogenous cellulase secreted from the midgut tissue has been considered a
A Brant et al.
Occupational and environmental medicine, 61(9), 793-795 (2004-08-20)
Three employees from two different detergent companies were investigated for occupational asthma, using skin prick tests, serum specific IgE, and specific bronchial challenge. Two were challenged with lipase and one with cellulase. All three cases had immunological evidence of sensitisation
Antonella Amore et al.
Microbial cell factories, 11, 164-164 (2012-12-27)
The use of lignocellulosic materials for second generation ethanol production would give several advantages such as minimizing the conflict between land use for food and fuel production, providing less expensive raw materials than conventional agricultural feedstock, allowing lower greenhouse gas
Katherine J Mackenzie et al.
Journal of the American Chemical Society, 135(1), 293-300 (2012-12-29)
Here we report the construction and characterization of a recoverable, thermoresponsive polymer-endoglucanase bioconjugate that matches the activity of unmodified enzymes on insoluble cellulose substrates. Two copolymers exhibiting a thermoresponsive lower critical solution temperature (LCST) were created through the copolymerization of
Wei Weiqi et al.
Bioresource technology, 128, 725-730 (2012-12-25)
Combination of liquid hot water pretreatment (LHWP) and wet disk milling (WDM) was investigated in this study to enhance the sugar recovery yield both in prehydrolyzate and enzymatic hydrolyzate. The results show that WDM with LHWP at 180 °C for
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