Anti-Chloride Channel-3 (CLC-3, Clcn3) antibody produced in rabbit

The protein contains a chloride channel (ClC) domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl^- ions across cell membranes, and the CBS domain may have a regulatory function.

highly purified GST fusion protein of the rat chloride channel-3 (CLC-3) corresponding to amino acids 592-661.

Anti-Chloride Channel-3 (CLC-3, Clcn3) antibody produced in rabbit is suitable for western blotting (chemiluminescent) at a working dilution of 1:200-1:300 using rat heart membranes and ECL. It was used as a primary antibody for western blotting at a dilution of 1:2000 to demonstrate adenoviral-mediated overexpression of ClC-3.

The encoded protein, voltage-gated chloride channel, is a transmembrane protein that maintains chloride ion homeostasis in various cells. It contains a chloride channel (ClC) domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl^- ions across cell membranes, and the CBS domain may have a regulatory function. ClC-3 deficiency inhibits Ang II-induced apoptosis of endothelial progenitor cells via suppressing ROS (reactive oxygen species) generation derived from NADPH oxidase. Osmotically active Cl^- is extruded via this channel upon kinase activation, which facilitates cytoplasmic condensation preceding cell division. ClC-3 protein may be considered as a potential tumor marker and therapeutic target for human nasopharyngeal carcinoma.

The epitope has significant homology with CLC-4 and CLC-5 proteins in rat and is well conserved in vertabrates.

Lyophilized at ~0.3 mg/ml from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, 5% sucrose, and 0.025% sodium azide.

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