C9658
羧肽酶 G 来源于假单胞菌 属
lyophilized powder, ≥3 units/mg protein
别名:
γ-谷氨酰水解酶
表单
lyophilized powder
质量水平
比活
≥3 units/mg protein
分子量
homodimer ~90 kDa
组成
Protein, ~70% biuret
溶解性
H2O: soluble 0.5 mg/mL
储存温度
−20°C
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应用
来自假单胞菌属或 γ-谷氨酰基水解酶的羧肽酶 G 已用于研究中评估假单孢氧还蛋白 COOH-末端在 P-450cam（细胞色素 m）羟基化中的作用。来源于假单胞菌属的羧肽酶 G 也被用于研究肺腺癌细胞中一氧化氮通过 NO‑cGMP 信号通路对培美曲塞细胞毒性的产生影响。
生化/生理作用
羧肽酶 G 是溶酶体，巯基依赖性蛋白酶，其逐渐切割 γ-谷氨酰蝶酰多聚-γ-谷氨酸，产生蝶酰-α-谷氨酸（叶酸）和游离谷氨酸。它被认为对 γ-谷氨酰基键具有高度特异性，但对于离去基团的 C-末端氨基酸则不具有高度特异性。该同型二聚体的分子量约为 90kDa。酶被 Zn2+ 离子激活。
外形
含有醋酸钠盐。
制备说明
溶液应现配现用。
色谱纯化产品
其他说明
在 pH7.3，30℃ 下，一个单位的酶每分钟将（+）氨甲蝶呤水解产生 1.0 μmole L-谷氨酸。
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Clearing up the confusion over the glutamate carboxypeptidase II gene.
Denise S O'Keefe et al.
American journal of medical genetics. Part A, 130A(3), 327-327 (2004-09-16)
Valentina Carozzi et al.
CNS & neurological disorders drug targets, 7(4), 348-360 (2008-11-11)
The role of Glutamate (Glu), one of the major excitatory neurotransmitters in the central nervous system, has been thoroughly investigated in animal models and in humans in several physiologic events, such as brain development and synaptic plasticity, but also in
Hiroki Nagai et al.
International journal of oncology, 41(1), 24-30 (2012-05-04)
Pemetrexed (PEM) is a novel, multitargeted, antifolate, antineoplastic agent for the treatment of non-small cell lung cancer and malignant pleural mesothelioma. Additional effects of nitric oxide (NO) donors on the chemosensitivity of cancers have been reported. However, the effects of
Klára Hlouchová et al.
Journal of neurochemistry, 101(3), 682-696 (2007-01-24)
Human glutamate carboxypeptidase II (GCPII) is a transmembrane metallopeptidase found mainly in the brain, small intestine, and prostate. In the brain, it cleaves N-acetyl-L-aspartyl-glutamate, liberating free glutamate. Inhibition of GCPII has been shown to be neuroprotective in models of stroke
S G Sligar et al.
Proceedings of the National Academy of Sciences of the United States of America, 71(10), 3906-3910 (1974-10-01)
Methylene hydroxylation by cytochrome P-450(cam) (cytochrome m) can be resolved into four distinct steps: substrate addition, m(o) --> m(os); reduction, m(os) --> m(rs); dioxygen addition, m(rs) --> m(O2) (rs); followed by a second putidaredoxin (Pseudomonas putida ferredoxin)-mediated reduction and product
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