C9748
Cellulase from Trichoderma longibrachiatum
powder, ≥1.0 unit/mg solid
form
powder
specific activity
≥1.0 unit/mg solid
composition
Protein, ≥10%
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
greener alternative category
storage temp.
room temp
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General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles.
Application
Cellulase from Trichoderma longibrachiatum has been used in a study to improve enzymatic saccharification. Cellulase from Trichoderma longibrachiatum has also been used in a study to investigate the effects of 2 exogenous pure cellulases on ruminal microbial growth and fermentation.
The enzyme has been used in the saccharification of biomass from energy cane during lignocellulosic ethanol production. It has been used in the enzymatic hydrolysis of treated and untreated wheat straw to develop an eco-friendly, dry alkaline chemo-mechanical pretreatment of wheat straw. The purpose of this process is to save energy input, to decrease the environmental impact and to increase enzymatic hydrolysis without production of waste and liquid fraction.
Biochem/physiol Actions
An acid cellulase with xylanase, pectinase, mannanase, xyloglucanase, laminarase, β-glucosidase, β-xylosidase, α-L-arabinofuranosidase, amylase, and protease activities
Other Notes
One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2 hr incubation time).
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
存储类别
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Yi Yang et al.
Microbial cell factories, 16(1), 166-166 (2017-09-28)
Xylan, the major constituent of hemicellulose, is composed of β-(1,4)-linked xylopyranosyl units that for the backbone, with side chains formed by other chemical moieties such as arabinose, galactose, mannose, ferulic acid and acetyl groups. Acetyl xylan esterases and α-L-arabinofuranosidases are
Yu Zhang et al.
International journal of molecular sciences, 21(2) (2020-01-16)
Fermentable sugars are important intermediate products in the conversion of lignocellulosic biomass to biofuels and other value-added bio-products. The main bottlenecks limiting the production of fermentable sugars from lignocellulosic biomass are the high cost and the low saccharification efficiency of
Eco-friendly dry chemo-mechanical pretreatments of lignocellulosic biomass: Impact on energy and yield of the enzymatic hydrolysis
Barakat A, et al.
Applied Energy, 113, 97-105 (2014)
Combined biological and chemical pretreatment method for lignocellulosic ethanol production from energy cane.
Suhardi VH, et al.
Renewable Bioresources, 1(1), 1-1 (2013)
Wen Kong et al.
Biotechnology for biofuels, 10, 218-218 (2017-09-20)
The selective lignin-degrading white-rot fungi are regarded to be the best lignin degraders and have been widely used for reducing the saccharification recalcitrance of lignocellulose. However, the biological delignification and conversion of lignocellulose in biorefinery is still limited. It is
实验方案
To standardize an enzymatic assay procedure of cellulase.
该实验方案使用分光光度法测定纤维素酶活性。黑曲霉纤维素酶催化内-1,4-β-D-糖苷键的水解。
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