Sf9 TitreHigh AC free
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
生物来源
insect ovary
包装
tube of 5 μg 05011001-DNA-5UG
pkg of vial of cells 05011001-1VL
生长模式
Adherent
核型
Not specified
形态学
Spherical
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
应用
Virus studies: Baculovirus
生化/生理作用
Spodoptera frugiperda pupal ovarian tissue, serum-free
This cell line is a derivative of the Sf9 cell line which has been adapted to TiterHigh Sf Insect Medium (Sigma I5408). TiterHigh Sf Insect Medium is an animal component-free, protein-free formulation designed specifically for the Sf9 (Spodoptera frugiperda) insect cell line. The cell line Sf9 is derived from pupal ovarian tissue of spodoptera frugiperda. The Sf9 cell line is highly susceptible to Baculovirus infection and is used in the production of protein products genetically manipulated into Baculovirus vector systems.
制备说明
Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 7.
TiterHigh™ Sf Insect Medium (Sigma I5408) no supplements.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法律信息
TiterHigh is a trademark of Sigma-Aldrich Co. LLC
法规信息
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