biological source
human kidney (embryonic)
packaging
tube of 5 μg 05011003-DNA-5UG, pkg of vial of cells 05011003-1VL
growth mode
Aggregates in suspension
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Human Embryo Kidney, serum-free
STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19
The human embryo kidney cell line 293 (Sigma Catalogue number. 85120602) adapted to grow in the animal component free medium Gene Therapy Medium 3 (Sigma G9916). This cell line grows in clumps in suspension. The original 293 cell line from which this cell line was derived was transformed with sheared human Ad5 DNA.
Preparation Note
Gene Therapy Medium 3 (Sigma G9916) + 2mM Glutamine. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.
Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran
Other Notes
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