biological source
mouse nerve
packaging
tube of 5 μg 08062552-DNA-5UG, pkg of vial of cells 08062552-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Neuronal
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Used in the generation of cell hybrids.
Biochem/physiol Actions
A bromodeoxyuridine-resistant mutant of N1E-115 (Sigma Catalogue number 08062511). The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300 (Mouse strain A/Jax)
Mouse neuroblastoma, bromodeoxyuridine resistant
Preparation Note
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridine.
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm? using 0.05% trypsin/EDTA; 5% CO2; 37?C.
Other Notes
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