Mouse malignant mesothelioma

Mice (BALB/c female 6-8 weeks) were exposed to crocidolite asbestos through intraperitoneal injection resulting in tumour development. Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic immunocompetent mice. Cell Bank Australia reference number: CBA0144

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 5% FCS

Optimal split ratio 1:8 (seeding density 1.2 x104 cells/cm²).Cells incubated at 37oC with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

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Depositor and originator: Richard Lake, University of Western Australia, Australia