AC29 cell line from mouse lung

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Mice (CBA strain female 6-8 weeks) were exposed to crocidiolite asbestos through intraperitoneal injection resulting in tumour development (both ascites and solid tumours). Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic imunocompetent mice. Cells grow as adherent monolayers and are epithelial like. Cell Bank Australia reference number: CBA0152

Mouse malignant mesothelioma

Optimal split ratio 1:8 - 1:16 (seeding density 0.6 x104 cells/cm²).Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

RPMI1640 (with 2mM L-Glutamine+ 25mM HEPES) + 5% FCS

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Richard Lake - University of Western Australia, Australia