Product Name
AC29 cell line from mouse lung, 10092308
生物来源
mouse lung
描述
Malignant mesothelioma from mouse lung
生长模式
Adherent
核型
Not specified
形态学
Epithelial-like
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Mouse malignant mesothelioma
细胞系描述
Mice (CBA strain female 6-8 weeks) were exposed to crocidiolite asbestos through intraperitoneal injection resulting in tumour development (both ascites and solid tumours). Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic imunocompetent mice. Cells grow as adherent monolayers and are epithelial like. Cell Bank Australia reference number: CBA0152
应用
Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.
制备说明
Optimal split ratio 1:8 - 1:16 (seeding density 0.6 x104 cells/cm2).Cells incubated at 37°C with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.
RPMI1640 (with 2mM L-Glutamine+ 25mM HEPES) + 5% FCS
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Richard Lake - University of Western Australia, Australia
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