PLC/PRF/5
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
别名:
Alexander cell line
生物来源
human liver
包装
tube of 5 μg 85061113-DNA-5UG
pkg of vial of cells 85061113-1VL
生长模式
Adherent
核型
Not specified
形态学
epithelial
产品
Hepatitis B Surface Antigen (HBSAg)
相关疾病
cancer
运输
dry ice
储存温度
−196°C
生化/生理作用
Alexander cell line which produces Hepatitis B antigen. The virus genome may be inducible in selective media. The cells should be handled under laboratory containment level 2. Expresses c-abl, c-fes, c-fms, c-myc, c-ha-ras and c-sis oncogenes. However there is currently no evidence that this cell line produces infectious Hepatitis B virus.
STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 13
D5S818: 12
D7S820: 9,11
THO1: 7,8
TPOX: 8
vWA: 15,16
Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 13
D5S818: 12
D7S820: 9,11
THO1: 7,8
TPOX: 8
vWA: 15,16
制备说明
DMEM + 2mM Glutamine + 10% fetal bovine serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6; i.e., seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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