biological source
Rat x Rat Hybridoma
packaging
tube of 5 μg 87072282-DNA-5UG, pkg of vial of cells 87072282-1VL
ImmunoDon
DA rat spleen
Myeloma
Y3Ag1.2.3
Reactivity
Anti-L3T4, mouse
technique(s)
cell culture | mammalian: suitable
isotype
IgG2b
shipped in
dry ice
storage temp.
−196°C
Immunogen
CBA mouse thymocytes
Biochem/physiol Actions
The antibody is directed against the A epitope of the mouse L3T4 T cell antigen, the murine equivalent of CD4. Has been used in in vivo depleting and flow cytometry studies. Cambridge Enterprise Reference number AB16: www.enterprise.cam.ac.uk.
Preparation Note
Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.
RPMI 1640 + 2 mM glutamine + 0.05 mM 2-mercaptoethanol (2ME) + 20% Fetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
法规信息
常规特殊物品
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