biological source
human skin
packaging
tube of 5 μg 88030401-DNA-5UG, pkg of vial of cells 88030401-1VL
growth mode
Adherent
karyotype
Triploid, modal n.o 69
morphology
Epithelial
products
Melanin
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Hormone studies
Biochem/physiol Actions
Established from a malignant melanoma of a 31 year old male Caucasian. The cells produce melanin for up to 50 population doublings. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Human Caucasian malignant melanoma
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 15
D5S818: 11
D7S820: 8,11
THO1: 7,9
TPOX: 8,10
vWA: 15,17
CSF1PO: 10
D13S317: 11
D16S539: 15
D5S818: 11
D7S820: 8,11
THO1: 7,9
TPOX: 8,10
vWA: 15,17
Preparation Note
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells are slow in attaching.
Other Notes
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法规信息
低风险生物材料
常规特殊物品
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