biological source
mouse unknown/unspecified
packaging
tube of 5 μg 89022402-DNA-5UG, pkg of vial of cells 89022402-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Please note: Available stocks of this cell line are not contact-inhibited. Contact inhibition is the natural process of arresting cell growth when cells come into contact with each other. It is not possible to be sure how the loss of contact inhibition may affect other characteristics of the cell line. This cell line catalog number continues to be a popular choice.
The key consideration is whether the characteristic of contact inhibition is required for cell line work. For example, it is required when testing the affects of expression of potential oncogenes where the induction of the loss of contact inhibition is being measured, whereas it is of less significance when using the cell line to express recombinant proteins for production and purification.
The key consideration is whether the characteristic of contact inhibition is required for cell line work. For example, it is required when testing the affects of expression of potential oncogenes where the induction of the loss of contact inhibition is being measured, whereas it is of less significance when using the cell line to express recombinant proteins for production and purification.
Mouse
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
低风险生物材料
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