生物来源
human skin
包装
tube of 5 μg 90011805-DNA-5UG
pkg of vial of cells 90011805-1VL
生长模式
Adherent
核型
Not specified
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
应用
DNA repair studies
生化/生理作用
Derived from a biopsy of a radiosensitive female. The cells have been used in cell survival studies.
Human skin fibroblast
制备说明
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他说明
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