receptors
Not specified
biological source
human skin
packaging
tube of 5 μg 90011807-DNA-5UG, pkg of vial of cells 90011807-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
DNA repair studies
Biochem/physiol Actions
Human skin fibroblast
Initiated on 6th November 1987 from a biopsy of a 51 year old normal male. The cells have been used in radiation survival studies. EBV-transformed lymphocytes designated LB182 (Sigma Catalogue number. 98062317) have been isolated from the same individual. This has been confirmed by STR profiling at ECACC.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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法规信息
低风险生物材料
常规特殊物品
此项目有
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