产品名称
161BR, 90011810
biological source
human skin
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
DNA repair studies
Biochem/physiol Actions
Established from a skin of a normal 49 year old male. The cells have been used in radiation survival studies.
Human skin fibroblast
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12,14
D16S539: 11
D5S818: 9,11
D7S820: 8,13
THO1: 6,9.3
TPOX: 8
vWA: 17,18
CSF1PO: 10,12
D13S317: 12,14
D16S539: 11
D5S818: 9,11
D7S820: 8,13
THO1: 6,9.3
TPOX: 8
vWA: 17,18
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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法规信息
常规特殊物品
低风险生物材料
此项目有
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