生物来源
human skin
包装
tube of 5 μg 90020507-DNA-5UG
pkg of vial of cells 90020507-1VL
生长模式
Adherent
核型
Not specified
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Human skin fibroblast, transformed
细胞系描述
Derived from a normal fibroblast (1BR3 Sigma Catalogue number. 90011801) transformed with the plasmid pSV3gpt.The genetic identity of 1BR3 (90011801), 1BR.3.G (90020507), 1BR.3.GN (90020508), and 1BR.3.N (90020509) has been confirmed by STR profiling at ECACC.
应用
DNA repair and cell growth studies
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他说明
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