WM-115

Tumourigenicity studies

Derived from a primary epitheloid tumour. Expresses proteoglycan antigen characteristic of melanomas. Tumourigenic in nude mice. See also WM 266-4 (Sigma Catalogue number 91061233) which is derived from a metastasis from the same patient.

Human melanoma

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12,13
D16S539: 11,12
D5S818: 13
D7S820: 8
THO1: 7,9
TPOX: 8,11
vWA: 15,17

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

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