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UNSPSC Code:
41106514
Biological source:
human skin (lymph node metastasis)
Relevant disease(s):
metastasis
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial
biological source
human skin (lymph node metastasis)
packaging
tube of 5 μg 91100402-DNA-5UG, pkg of vial of cells 91100402-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Nerve growth factor (NGF), Laminin
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Human caucasian metastatic melanoma
This cell line has been isolated from a lymph node metastasis from a 43 year old male patient with malignant melanoma. Due to their highly invasive properties, the cells can be used as a source of cellular invasion associated proteins e.g. collagen type IV collagenase, tissue inhibitor of metalloproteinase-2 and autocrine motility factor.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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法规信息
常规特殊物品
低风险生物材料
此项目有
商品
DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
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