143B
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91112502, human bone, Not specified
生物来源
human bone
包装
tube of 5 μg 91112502-DNA-5UG
pkg of vial of cells 91112502-1VL
生长模式
Adherent
核型
Not specified
形态学
Not specified
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Tumourigenicity studies
生化/生理作用
Cells are thymidine kinase deficient (TK-) and resistant to BUdR.143B and HOS (Sigma Catalogue number 87070202) originated from the same host: 143B is TK- and originated indirectly from HOS which is TK+.
Human bone osteosarcoma TK-
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18
制备说明
EMEM (EBSS) + 2mM Glutamine + 15ug/ml Bromo-deoxy Uridine (BUdR) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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