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Merck
CN

46Br.1G1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92091814, human skin, Fibroblast

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关于此项目

UNSPSC Code:
41106514
Biological source:
human skin
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Fibroblast

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biological source

human skin

packaging

tube of 5 μg 92091814-DNA-5UG, pkg of vial of cells 92091814-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

相关类别

Biochem/physiol Actions

Derived from 46BR, a fibroblast from an individual with hypogammaglobulinaemia. The parent line was transformed with the plasmid pSV3gpt expressing SV40 T-antigen.
Human skin fibroblast, SV40 T antigen transformed

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin; EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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低风险生物材料
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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