生物来源
human skin
包装
tube of 5 μg 92091814-DNA-5UG
pkg of vial of cells 92091814-1VL
生长模式
Adherent
核型
Not specified
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
生化/生理作用
Derived from 46BR, a fibroblast from an individual with hypogammaglobulinaemia. The parent line was transformed with the plasmid pSV3gpt expressing SV40 T-antigen.
Human skin fibroblast, SV40 T antigen transformed
制备说明
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin; EDTA; 5% CO2; 37°C.
其他说明
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法规信息
新产品
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