Mouse melanoma, producing melanin

Mouse melanoma which produces melanin.

B16-F1 cell line from mouse has been used to study the cytotoxic and antioxidant properties of supercritical CO2 extract (SCE) of old man′s beard (Usnea barbata). It has also been used to study the antiproliferative activity of essential oils from Achillea ligustica.

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

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