生物来源
human kidney
包装
tube of 5 μg 93020513-DNA-5UG
pkg of vial of cells 93020513-1VL
生长模式
Adherent
核型
2n = 46, aneuploid
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Human kidney adenocarcinoma
细胞系描述
Derived from 78-year-old male. Does not produce tumours in nude mice.
应用
Tumourigenicity studies
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x100,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他说明
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