biological source
human esophagus
packaging
tube of 5 μg 94072023-DNA-5UG, pkg of vial of cells 94072023-1VL
growth mode
Adherent
karyotype
Hypotetraploid with 4% polyploidy
morphology
Epitheloid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Established from the poorly differentiated invasive oesophageal squamous cell carcinoma resected from the cervical oesophagus of a 51-year old Japanese man prior to treatment. Reported to overexpress heparin binding growth factor (hst-1) and cyclin D. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.
Human oesophageal squamous cell carcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 10,12
D5S818: 13
D7S820: 12
THO1: 8
TPOX: 8,11
vWA: 16,18
CSF1PO: 12
D13S317: 11
D16S539: 10,12
D5S818: 13
D7S820: 12
THO1: 8
TPOX: 8,11
vWA: 16,18
Preparation Note
RPMI 1640 + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS)
Split confluent cultures 1:5 to 1:10 twice a week i.e. seeding at 2-4x10,000 cells/cm2 using trypsin/EDTA;5% CO2; 37°C. Doubling time ~45h
Other Notes
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法规信息
常规特殊物品
低风险生物材料
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