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UNSPSC Code:
41106514
Biological source:
rat prostate
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
44XY, +4, + 12 del(3)(3q32-36)del(15)p14
Morphology:
Epithelial
biological source
rat prostate
packaging
tube of 5 μg 94101452-DNA-5UG, pkg of vial of cells 94101452-1VL
growth mode
Adherent
karyotype
44XY, +4, + 12 del(3)(3q32-36)del(15)p14
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Used in studies in which micro-cell mediated chromosomal transfer of human chromosome 11 suppressed metastatic ability. This suppresser function was localised to 11p11.2-13.
Biochem/physiol Actions
A highly metastatic, anaplastic, androgen-independent sub-line which spontaneously developed during the serial passage of the non-metastatic, well-differentiated, androgen-responsive R-3327-H sub-lines in the laboratory of Dr J Schalken, Nijmegan, The Netherlands.
Rat dorsal prostatic adenocarcinoma
Preparation Note
RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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法规信息
低风险生物材料
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