生物来源
rat liver
包装
tube of 5 μg 94101909-DNA-5UG
pkg of vial of cells 94101909-1VL
生长模式
Adherent
核型
100 chromosomes
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Gene analysis and liver function expression.
生化/生理作用
HF1-5 is a subclone of HF1 selected for it′s ability to grow in glucose free medium. The entire group of hepatic functions are re-expressed. NB. The cells are difficult to cultivate, they do not like either low or high density and should be transferred frequently even though they grow slowly.
Rat hepatoma Hybrid [H5xFao]
制备说明
Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Split semi-confluent (60%-70%) cultures 1:5 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 37°C. Saturation density is reached at 5x1000,000 cells/10cm dish.
其他说明
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