生物来源
rat liver
包装
tube of 5 μg 94101909-DNA-5UG
pkg of vial of cells 94101909-1VL
生长模式
Adherent
核型
100 chromosomes
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Rat hepatoma Hybrid [H5xFao]
细胞系描述
HF1-5 is a subclone of HF1 selected for it′s ability to grow in glucose free medium. The entire group of hepatic functions are re-expressed. NB. The cells are difficult to cultivate, they do not like either low or high density and should be transferred frequently even though they grow slowly.
应用
Gene analysis and liver function expression.
培养基
Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
传代培养常规
Split semi-confluent (60%-70%) cultures 1:5 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 37°C. Saturation density is reached at 5x1000,000 cells/10cm dish.
其他说明
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