biological source
rat liver
packaging
tube of 5 μg 94101909-DNA-5UG, pkg of vial of cells 94101909-1VL
growth mode
Adherent
karyotype
100 chromosomes
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Gene analysis and liver function expression.
Biochem/physiol Actions
HF1-5 is a subclone of HF1 selected for it′s ability to grow in glucose free medium. The entire group of hepatic functions are re-expressed. NB. The cells are difficult to cultivate, they do not like either low or high density and should be transferred frequently even though they grow slowly.
Rat hepatoma Hybrid [H5xFao]
Preparation Note
Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Split semi-confluent (60%-70%) cultures 1:5 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 37°C. Saturation density is reached at 5x1000,000 cells/10cm dish.
Other Notes
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法规信息
低风险生物材料
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